[skeptic]

18 – 23.07.10 в Вене проходила XVIII Международная конференция по проблемам ВИЧ/СПИДа. [Ссылки доступны только зарегистрированным пользователям ] [Ссылки доступны только зарегистрированным пользователям ] На сайте конференции из примерно трёх десятков докладов, посвящённых созданию анти-ВИЧ вакцин лишь один был представлен из России, точнее, из ГНЦ вирусологии и биотехнологии «Вектор» Роспотребнадзора (Новосибирск). Докладчик – А. Чикаев был удостоен стипендии для поддержки проводимых исследований. Вот абстракт:
MOPE0042 Recombinant proteins carrying peptide mimics recognized by broadly neutralizing anti-HIV-1 mAb 2G12 as a candidate vaccine A. Chikaev, N. Shcherbakova, O. Tumanova, L. Karpenko, A. Ilyichev State Research Center of Virology and Biotechnology VECTOR,
Immunotherapeutic drugs Department, Novosibirsk, Russian Federation
Background: An AIDS vaccine is unlikely without a solution to the HIV neutralizing antibody problem. An artifi cial polyepitope protein TBI (T and B cell epitopes containing immunogen) was created in our institute to this purpose. It contains 4 T-cellular and 5 B-cellular linear neutralizing epitopes from Env and Gag HIV-1 proteins. Immunization of monkeys and mice by such protein elicited HIV-specifi c immune response.
Methods: MAb 2G12 neutralizes HIV-1 by binding with high affi nity to a cluster of high-mannose oligosaccharides on the envelope glycoprotein, gp120.It was decided to improve polyepitope protein TBI including in its structure mimic of conformational antigenic determinants recognized by 2G12 mAb. Phage display was used to obtain peptides mimicking a HIV-1 gp120 conformational epitope recognized by virus-neutralizing mAb 2G12. The oligonucleotides, coding for the 3 selected peptide mimics were synthesized and cloned into the TBI gene. Balb/c mice were immunized with the new recombinant TBI proteins.
Results: We have obtained 3 recombinant TBI proteins containing peptide mimics recognized by 2G12. The antigenic properties of recombinant TBI proteins were characterized by immunoblot and ELISA. Antibodies to gp120 were detected in the sera of immunized animals. The virus-neutralizing activity of the sera were examined.
Conclusions: Thus, new recombinant TBI proteins which possess antigenicityand immunogenicity of peptide mimics of 2G12 mAb were obtained. These proteins are expected to be promising as a component of HIV vaccine.
Presenting author email: anch09@mail. ru [Ссылки доступны только зарегистрированным пользователям ] Как видите, уважаемые читатели, это – хорошая экспериментальная работа – идейная, так сказать, предпосылка для попытки создать вакцину, но о реальной вакцине летом 2010, три месяца назад, речи не было и быть не могло.