[Dr.Vad]

Compared with the control condition — overnight fasting followed by a morning iron dose taken with water three hours before coffee or breakfast
—
a morning dose taken with either 80 mg or 500 mg of ascorbic acid significantly improved iron absorption by about 30%.

The higher dose did not yield significantly higher absorption.

All other strategies reduced iron absorption:
afternoon dosing by 37%;
coadministration with coffee in the morning by 54%;
administration in the morning with coffee, 90 mg of ascorbic acid, and food by 66%.

Compared to consuming a 100 mg iron dose in the morning with coffee or breakfast, consuming it with orange juice alone results in a ~ 4-fold increase in iron absorption, and provides ~20 more mg of absorbed iron per dose.

Effect of dietary factors and time of day on iron absorption from oral iron supplements in iron deficient women
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