Полагаю, что две работы, приведенные ниже, дают дополнительную информацию к ряду вопросов, поднятых в наших длительных дискуссиях с уважаемым Евгений Евгеньевичем.
Artificial Organs
Volume 28 Issue 2 Page 234 - February 2004
doi:10.1111/j.1525-1594.2003.47211.x
Ozonated Autohemotherapy in Patients on Maintenance Hemodialysis: Influence on Lipid Profile and Endothelium
*Leszek Tylicki, *Bogdan Biedunkiewicz, *Tomasz Nieweglowski, *Andrzej Chamienia, *Alicja Debska Slizien, Joanna Luty, Wieslawa Lysiak-Szydlowska, and *Boleslaw Rutkowski
Abstract:
Ozonated autohemotherapy (O3-AHT) is used in the treatment of atherosclerotic ischemia of lower limbs (AILL). The impact of ozone on serum lipids and endothelium injury is of particular interest since these factors are important in the development of atherosclerotic lesions. To evaluate this issue, a prospective, placebo-controlled study was designed. Twelve hemodialyzed subjects with AILL received autohemotherapy with oxygen as a control followed by O3-AHT with ozone concentration of 50 µg/ml. Serum lipids and plasma activity of von Willebrand factor (vWF) were measured. After O3-AHT, total cholesterol significantly decreased compared to the baseline ( 8.34%) [P < 0.01]. LDL cholesterol was also significantly lower than the initial value ( 17.71%) [P < 0.001]. No significant changes in the activity of vWF were found after the first session of O3-AHT and after all nine sessions of O3-AHT. The study demonstrated that O3-AHT did not affect deleteriously the endothelium in patients with chronic renal failure on maintenance hemodialysis. It may stimulate beneficial changes in serum lipid profile manifesting as a decrease in the total- and LDL-cholesterol levels.
Liver International
Volume 24 Issue 1 Page 55 - February 2004
doi:10.1111/j.1478-3231.2004.00885.x
Effects of ozone oxidative preconditioning on nitric oxide generation and cellular redox balance in a rat model of hepatic ischaemia-reperfusion
H. H. Ajamieh1, S. Menéndez2, G. Martínez-Sánchez1, E. Candelario-Jalil1, L. Re3, A. Giuliani4 and Olga Sonia León Fernández1
Abstract:
Background: Many studies indicate that oxygen free-radical formation after reoxygenation of liver may initiate the cascade of hepatocellular injury. It has been demonstrated that controlled ozone administration may promote an oxidative preconditioning or adaptation to oxidative stress, preventing the damage induced by reactive oxygen species and protecting against liver ischaemia-reperfusion (I/R) injury.
Aims: In the present study, the effects of ozone oxidative preconditioning (OzoneOP) on nitric oxide (NO) generation and the cellular redox balance have been studied.
Methods: Six groups of rats were classified as follows: (1) sham-operated; (2) sham-operated+L-NAME (N -nitro-L-arginine methyl ester); (3) I/R (ischaemia 90 min-reperfusion 90 min); (4) OzoneOP+I/R; (5) OzoneOP+L-NAME+I/R; and (6) L-NAME+I/R. The following parameters were measured: plasma transaminases (aspartate aminotransferase, alanine aminotransferase) as an index of hepatocellular injury; in homogenates of hepatic tissue: nitrate/nitrite as an index of NO production; superoxide dismutase (SOD), catalase (CAT) and glutathione levels as markers of endogenous antioxidant system; and finally malondialdehyde+4-hydroxyalkenals (MDA+4-HDA) and total hydroperoxides (TH) as indicators of oxidative stress.
Results: A correspondence between liver damage and the increase of NO, CAT, TH, glutathione and MDA+4-HDA concentrations were observed just as a decrease of SOD activity. OzoneOP prevented and attenuated hepatic damage in I/R and OzoneOP+L-NAME+I/R, respectively, in close relation with the above-mentioned parameters.
Conclusions: These results show that OzoneOP protected against liver I/R injury through mechanisms that promote a regulation of endogenous NO concentrations and maintenance of cellular redox balance. Ozone treatment may have important clinical implications, particularly in view of the increasing hepatic transplantation programs
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